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Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain the transc...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405533/ https://www.ncbi.nlm.nih.gov/pubmed/36035800 http://dx.doi.org/10.1016/j.xpro.2022.101633 |