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Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain

Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain the transc...

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Detalles Bibliográficos
Autores principales: Yamamoto, Hajime, Abe, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405533/
https://www.ncbi.nlm.nih.gov/pubmed/36035800
http://dx.doi.org/10.1016/j.xpro.2022.101633