Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain

Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain the transc...

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Detalles Bibliográficos
Autores principales: Yamamoto, Hajime, Abe, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405533/
https://www.ncbi.nlm.nih.gov/pubmed/36035800
http://dx.doi.org/10.1016/j.xpro.2022.101633
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author Yamamoto, Hajime
Abe, Kentaro
author_facet Yamamoto, Hajime
Abe, Kentaro
author_sort Yamamoto, Hajime
collection PubMed
description Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain the transcription factor activity profile (TFAP). Our protocol facilitates a systematic analysis of TF activity of the brain in vivo and will aid trans-omics understanding of the molecular mechanism underlying the brain functions. For complete details on the use and execution of this protocol, please refer to Abe and Abe, (2022).
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spelling pubmed-94055332022-08-26 Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain Yamamoto, Hajime Abe, Kentaro STAR Protoc Protocol Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain the transcription factor activity profile (TFAP). Our protocol facilitates a systematic analysis of TF activity of the brain in vivo and will aid trans-omics understanding of the molecular mechanism underlying the brain functions. For complete details on the use and execution of this protocol, please refer to Abe and Abe, (2022). Elsevier 2022-08-18 /pmc/articles/PMC9405533/ /pubmed/36035800 http://dx.doi.org/10.1016/j.xpro.2022.101633 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Yamamoto, Hajime
Abe, Kentaro
Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title_full Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title_fullStr Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title_full_unstemmed Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title_short Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
title_sort protocol for viral vector-mediated measurement of transcription factor activity of mouse brain
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405533/
https://www.ncbi.nlm.nih.gov/pubmed/36035800
http://dx.doi.org/10.1016/j.xpro.2022.101633
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