A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryoprese...

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Detalles Bibliográficos
Autores principales: Knittel, Jacob, Srinivasan, Gayathri, Frisch, Carlye, Brookhouser, Nicholas, Raman, Sreedevi, Essuman, Albert, Brafman, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405537/
https://www.ncbi.nlm.nih.gov/pubmed/36035791
http://dx.doi.org/10.1016/j.xpro.2022.101632

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405537/
https://www.ncbi.nlm.nih.gov/pubmed/36035791
http://dx.doi.org/10.1016/j.xpro.2022.101632

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