A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryoprese...

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Detalles Bibliográficos
Autores principales: Knittel, Jacob, Srinivasan, Gayathri, Frisch, Carlye, Brookhouser, Nicholas, Raman, Sreedevi, Essuman, Albert, Brafman, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405537/
https://www.ncbi.nlm.nih.gov/pubmed/36035791
http://dx.doi.org/10.1016/j.xpro.2022.101632
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author Knittel, Jacob
Srinivasan, Gayathri
Frisch, Carlye
Brookhouser, Nicholas
Raman, Sreedevi
Essuman, Albert
Brafman, David A.
author_facet Knittel, Jacob
Srinivasan, Gayathri
Frisch, Carlye
Brookhouser, Nicholas
Raman, Sreedevi
Essuman, Albert
Brafman, David A.
author_sort Knittel, Jacob
collection PubMed
description Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).
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spelling pubmed-94055372022-08-26 A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes Knittel, Jacob Srinivasan, Gayathri Frisch, Carlye Brookhouser, Nicholas Raman, Sreedevi Essuman, Albert Brafman, David A. STAR Protoc Protocol Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021). Elsevier 2022-08-18 /pmc/articles/PMC9405537/ /pubmed/36035791 http://dx.doi.org/10.1016/j.xpro.2022.101632 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Knittel, Jacob
Srinivasan, Gayathri
Frisch, Carlye
Brookhouser, Nicholas
Raman, Sreedevi
Essuman, Albert
Brafman, David A.
A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title_full A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title_fullStr A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title_full_unstemmed A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title_short A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
title_sort microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9405537/
https://www.ncbi.nlm.nih.gov/pubmed/36035791
http://dx.doi.org/10.1016/j.xpro.2022.101632
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