PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

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Detalles Bibliográficos
Autores principales: Yuan, Guoliang, Martin, Stanton, Hassan, Md Mahmudul, Tuskan, Gerald A., Yang, Xiaohan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406951/
https://www.ncbi.nlm.nih.gov/pubmed/36010544
http://dx.doi.org/10.3390/cells11162467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9406951/
https://www.ncbi.nlm.nih.gov/pubmed/36010544
http://dx.doi.org/10.3390/cells11162467

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