Cargando…

Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing

Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or food poisoning due to its toxigenic and pathogenic nature. In this study, we identified members of B. cereus groups in 65% of the ice cream samples analyzed, which were characterized based on multi locus vari...

Descripción completa

Detalles Bibliográficos
Autores principales: Fraccalvieri, Rosa, Bianco, Angelica, Difato, Laura Maria, Capozzi, Loredana, Del Sambro, Laura, Simone, Domenico, Catanzariti, Roberta, Caruso, Marta, Galante, Domenico, Normanno, Giovanni, Palazzo, Lucia, Tempesta, Maria, Parisi, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407285/
https://www.ncbi.nlm.nih.gov/pubmed/36010481
http://dx.doi.org/10.3390/foods11162480
_version_ 1784774327438147584
author Fraccalvieri, Rosa
Bianco, Angelica
Difato, Laura Maria
Capozzi, Loredana
Del Sambro, Laura
Simone, Domenico
Catanzariti, Roberta
Caruso, Marta
Galante, Domenico
Normanno, Giovanni
Palazzo, Lucia
Tempesta, Maria
Parisi, Antonio
author_facet Fraccalvieri, Rosa
Bianco, Angelica
Difato, Laura Maria
Capozzi, Loredana
Del Sambro, Laura
Simone, Domenico
Catanzariti, Roberta
Caruso, Marta
Galante, Domenico
Normanno, Giovanni
Palazzo, Lucia
Tempesta, Maria
Parisi, Antonio
author_sort Fraccalvieri, Rosa
collection PubMed
description Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or food poisoning due to its toxigenic and pathogenic nature. In this study, we identified members of B. cereus groups in 65% of the ice cream samples analyzed, which were characterized based on multi locus variable number tandem repeats analysis (MLVA) and whole genome sequencing (WGS). The MLVA revealed that 36 strains showed different allelic profiles. Analyses of WGS data enabled the identification of three members of the B. cereus group: B. cereus sensu stricto, B. mosaicus and B. thuringiensis. Based on the multi locus sequence typing (MLST) scheme, the strains were classified in 27 sequence types (STs), including ST26 that causes food poisoning. Toxin genes’ detection revealed the presence of the genes encoding nonhemolytic enterotoxin (NHE), hemolysin BL (HBL), cytotoxin K (cytK) and cereulide (ces) in 100%, 44%, 42% and 8% of the strains, respectively. The identification of eleven antimicrobial resistance (AMR) genes predicted the resistance to five different antimicrobials, and the resistance to beta-lactam antibiotics was confirmed with a phenotypic antimicrobial test. Taken together, the results showed that the B. cereus strains isolated from ice cream were a potential hazard for consumer safety.
format Online
Article
Text
id pubmed-9407285
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94072852022-08-26 Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing Fraccalvieri, Rosa Bianco, Angelica Difato, Laura Maria Capozzi, Loredana Del Sambro, Laura Simone, Domenico Catanzariti, Roberta Caruso, Marta Galante, Domenico Normanno, Giovanni Palazzo, Lucia Tempesta, Maria Parisi, Antonio Foods Article Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or food poisoning due to its toxigenic and pathogenic nature. In this study, we identified members of B. cereus groups in 65% of the ice cream samples analyzed, which were characterized based on multi locus variable number tandem repeats analysis (MLVA) and whole genome sequencing (WGS). The MLVA revealed that 36 strains showed different allelic profiles. Analyses of WGS data enabled the identification of three members of the B. cereus group: B. cereus sensu stricto, B. mosaicus and B. thuringiensis. Based on the multi locus sequence typing (MLST) scheme, the strains were classified in 27 sequence types (STs), including ST26 that causes food poisoning. Toxin genes’ detection revealed the presence of the genes encoding nonhemolytic enterotoxin (NHE), hemolysin BL (HBL), cytotoxin K (cytK) and cereulide (ces) in 100%, 44%, 42% and 8% of the strains, respectively. The identification of eleven antimicrobial resistance (AMR) genes predicted the resistance to five different antimicrobials, and the resistance to beta-lactam antibiotics was confirmed with a phenotypic antimicrobial test. Taken together, the results showed that the B. cereus strains isolated from ice cream were a potential hazard for consumer safety. MDPI 2022-08-17 /pmc/articles/PMC9407285/ /pubmed/36010481 http://dx.doi.org/10.3390/foods11162480 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fraccalvieri, Rosa
Bianco, Angelica
Difato, Laura Maria
Capozzi, Loredana
Del Sambro, Laura
Simone, Domenico
Catanzariti, Roberta
Caruso, Marta
Galante, Domenico
Normanno, Giovanni
Palazzo, Lucia
Tempesta, Maria
Parisi, Antonio
Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title_full Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title_fullStr Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title_full_unstemmed Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title_short Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
title_sort toxigenic genes, pathogenic potential and antimicrobial resistance of bacillus cereus group isolated from ice cream and characterized by whole genome sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407285/
https://www.ncbi.nlm.nih.gov/pubmed/36010481
http://dx.doi.org/10.3390/foods11162480
work_keys_str_mv AT fraccalvierirosa toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT biancoangelica toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT difatolauramaria toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT capozziloredana toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT delsambrolaura toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT simonedomenico toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT catanzaritiroberta toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT carusomarta toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT galantedomenico toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT normannogiovanni toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT palazzolucia toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT tempestamaria toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing
AT parisiantonio toxigenicgenespathogenicpotentialandantimicrobialresistanceofbacilluscereusgroupisolatedfromicecreamandcharacterizedbywholegenomesequencing