Influence of Unequal Amplification of Methylated and Non-Methylated Template on Performance of Pyrosequencing

Pyrosequencing is one of the technologies widely used for quantitative methylation assessment. The protocol of pyrosequencing experiment consists of PCR amplification of a locus of interest and subsequent sequencing via synthesis of the amplified PCR product. As the PCR in this protocol utilizes one...

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Detalles Bibliográficos
Autores principales: Taryma-Lesniak, Olga, Kjeldsen, Tina E., Hansen, Lise Lotte, Wojdacz, Tomasz K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407541/
https://www.ncbi.nlm.nih.gov/pubmed/36011328
http://dx.doi.org/10.3390/genes13081418

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407541/
https://www.ncbi.nlm.nih.gov/pubmed/36011328
http://dx.doi.org/10.3390/genes13081418

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