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Influence of Unequal Amplification of Methylated and Non-Methylated Template on Performance of Pyrosequencing
Pyrosequencing is one of the technologies widely used for quantitative methylation assessment. The protocol of pyrosequencing experiment consists of PCR amplification of a locus of interest and subsequent sequencing via synthesis of the amplified PCR product. As the PCR in this protocol utilizes one...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407541/ https://www.ncbi.nlm.nih.gov/pubmed/36011328 http://dx.doi.org/10.3390/genes13081418 |