Quantitative Evaluation of Very Low Levels of HIV-1 Reverse Transcriptase by a Novel Highly Sensitive RT-qPCR Assay

Based on previous experience in our laboratory, we developed a real-time reverse transcriptase (RT) quantitative PCR (RT-qPCR) assay for the assessment of very low levels of HIV-1 RT activity. The RNA, acting as a template for reverse transcription into cDNA by HIV-1 RT, consisted of a synthetic RNA...

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Detalles Bibliográficos
Autores principales: Marino-Merlo, Francesca, Stefanizzi, Valeria, Ragno, Agnese, Piredda, Lucia, Grelli, Sandro, Macchi, Beatrice, Mastino, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410348/
https://www.ncbi.nlm.nih.gov/pubmed/36013309
http://dx.doi.org/10.3390/life12081130

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410348/
https://www.ncbi.nlm.nih.gov/pubmed/36013309
http://dx.doi.org/10.3390/life12081130

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