Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs

Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole metabolic pathways into the genome. This approach r...

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Detalles Bibliográficos
Autores principales: Bubnov, Dmitrii M, Yuzbashev, Tigran V, Khozov, Andrey A, Melkina, Olga E, Vybornaya, Tatiana V, Stan, Guy-Bart, Sineoky, Sergey P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410887/
https://www.ncbi.nlm.nih.gov/pubmed/35920321
http://dx.doi.org/10.1093/nar/gkac649

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410887/
https://www.ncbi.nlm.nih.gov/pubmed/35920321
http://dx.doi.org/10.1093/nar/gkac649

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