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Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs
Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole metabolic pathways into the genome. This approach r...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410887/ https://www.ncbi.nlm.nih.gov/pubmed/35920321 http://dx.doi.org/10.1093/nar/gkac649 |