Inducible expression of large gRNA arrays for multiplexed CRISPRai applications

CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering. Here we develop a method for near leak-free, inducible expression of a polycistronic array...

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Detalles Bibliográficos
Autores principales: Shaw, William M., Studená, Lucie, Roy, Kyler, Hapeta, Piotr, McCarty, Nicholas S., Graham, Alicia E., Ellis, Tom, Ledesma-Amaro, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411621/
https://www.ncbi.nlm.nih.gov/pubmed/36008396
http://dx.doi.org/10.1038/s41467-022-32603-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411621/
https://www.ncbi.nlm.nih.gov/pubmed/36008396
http://dx.doi.org/10.1038/s41467-022-32603-7

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