Iodixanol density gradients as an effective phytoplasma enrichment approach to improve genome sequencing

Obtaining complete phytoplasma genomes is difficult due to the lack of a culture system for these bacteria. To improve genome assembly, a non-ionic, low- and iso-osmotic iodixanol (Optiprep™) density gradient centrifugation method was developed to enrich for phytoplasma cells and deplete plant host...

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Detalles Bibliográficos
Autores principales: Rodrigues Jardim, Bianca, Tran-Nguyen, Lucy T. T., Gambley, Cherie, Rodoni, Brendan, Constable, Fiona E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411968/
https://www.ncbi.nlm.nih.gov/pubmed/36033837
http://dx.doi.org/10.3389/fmicb.2022.937648

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9411968/
https://www.ncbi.nlm.nih.gov/pubmed/36033837
http://dx.doi.org/10.3389/fmicb.2022.937648

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