A simple strategy for addition of degron tags to endogenous genes harboring prior insertions of fluorescent protein.

There exist insufficient validated “entry portal” sites in the C. elegans genome for CRISPR/Cas9-dependent insertion into endogenous genes to confer diverse spatiotemporal patterns and levels of expression on exogenous sequences. Consequently, we recognized the most common potential “entry portal” s...

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Detalles Bibliográficos
Autores principales: Fakieh, Razan, Duong, Tam, Wu, You, Rasmussen, Neal, Reiner, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412190/
https://www.ncbi.nlm.nih.gov/pubmed/36035777
http://dx.doi.org/10.17912/micropub.biology.000622

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412190/
https://www.ncbi.nlm.nih.gov/pubmed/36035777
http://dx.doi.org/10.17912/micropub.biology.000622

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