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A simple strategy for addition of degron tags to endogenous genes harboring prior insertions of fluorescent protein.
There exist insufficient validated “entry portal” sites in the C. elegans genome for CRISPR/Cas9-dependent insertion into endogenous genes to confer diverse spatiotemporal patterns and levels of expression on exogenous sequences. Consequently, we recognized the most common potential “entry portal” s...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Caltech Library
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9412190/ https://www.ncbi.nlm.nih.gov/pubmed/36035777 http://dx.doi.org/10.17912/micropub.biology.000622 |