An in vitro reconstitution system to monitor iron transfer to the active site during the maturation of [NiFe]-hydrogenase

[NiFe]-hydrogenases (Hyds) comprise a small and a large subunit. The latter harbors the biologically unique [NiFe](CN)(2)CO active-site cofactor. The maturation process includes the assembly of the [Fe](CN)(2)CO cofactor precursor, nickel binding, endoproteolytic cleavage of the large subunit, and d...

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Detalles Bibliográficos
Autores principales: Soboh, Basem, Adrian, Lorenz, Stripp, Sven T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418501/
https://www.ncbi.nlm.nih.gov/pubmed/35868564
http://dx.doi.org/10.1016/j.jbc.2022.102291

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418501/
https://www.ncbi.nlm.nih.gov/pubmed/35868564
http://dx.doi.org/10.1016/j.jbc.2022.102291

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