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An in vitro reconstitution system to monitor iron transfer to the active site during the maturation of [NiFe]-hydrogenase
[NiFe]-hydrogenases (Hyds) comprise a small and a large subunit. The latter harbors the biologically unique [NiFe](CN)(2)CO active-site cofactor. The maturation process includes the assembly of the [Fe](CN)(2)CO cofactor precursor, nickel binding, endoproteolytic cleavage of the large subunit, and d...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Biochemistry and Molecular Biology
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9418501/ https://www.ncbi.nlm.nih.gov/pubmed/35868564 http://dx.doi.org/10.1016/j.jbc.2022.102291 |