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Glucose oxidase kinetics using MnO(2) nanosheets: confirming Michaelis–Menten kinetics and quantifying decreasing enzyme performance with increasing buffer concentration

MnO(2) nanosheets and ultraviolet-visible (UV-Vis) absorbance spectroscopy are used to study glucose oxidase (GOx) kinetics. Glucose oxidation by GOx produces H(2)O(2), which rapidly decomposes the nanosheets and reduces their absorption. This direct approach for monitoring glucose oxidation enables...

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Detalles Bibliográficos
Autores principales: Singh, Mahip, Ungku Faiz, Ungku Zoe Anysa, Gravelsins, Steven, Suganuma, Yoshinori, Kotoulas, Nicholas Konstantine, Croxall, Mark, Khan-Trottier, Ahlia, Goh, Cynthia, Dhirani, Al-Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9419709/
https://www.ncbi.nlm.nih.gov/pubmed/36133026
http://dx.doi.org/10.1039/d1na00311a