Exploiting CRISPR/Cas9 to engineer precise segmental deletions in mouse embryonic stem cells

In this protocol, we use CRISPR/Cas9 to generate large deletions of the entire coding region of a gene of interest, generating a hemizygous cell line. Next, we systematically engineer precise in-frame deletions within the intact wild-type allele, facilitating study of multi-domain proteins. The opti...

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Detalles Bibliográficos
Autores principales: Elango, Rajula, Panday, Arvind, Willis, Nicholas A., Scully, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420392/
https://www.ncbi.nlm.nih.gov/pubmed/36042887
http://dx.doi.org/10.1016/j.xpro.2022.101551

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420392/
https://www.ncbi.nlm.nih.gov/pubmed/36042887
http://dx.doi.org/10.1016/j.xpro.2022.101551

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