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Exploiting CRISPR/Cas9 to engineer precise segmental deletions in mouse embryonic stem cells
In this protocol, we use CRISPR/Cas9 to generate large deletions of the entire coding region of a gene of interest, generating a hemizygous cell line. Next, we systematically engineer precise in-frame deletions within the intact wild-type allele, facilitating study of multi-domain proteins. The opti...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420392/ https://www.ncbi.nlm.nih.gov/pubmed/36042887 http://dx.doi.org/10.1016/j.xpro.2022.101551 |