Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states

RNA helicase DDX21 plays vital roles in ribosomal RNA processing and the regulation of host innate immunity during virus infection. Here, we describe the optimized protocols for nucleic acid-free protein purification and crystallization of DDX21 in its different unwinding states. Rational design of...

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Detalles Bibliográficos
Autores principales: Chen, Zijun, Huang, Jinqing, Li, Jixi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420396/
https://www.ncbi.nlm.nih.gov/pubmed/36042885
http://dx.doi.org/10.1016/j.xpro.2022.101642

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420396/
https://www.ncbi.nlm.nih.gov/pubmed/36042885
http://dx.doi.org/10.1016/j.xpro.2022.101642

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