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Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states
RNA helicase DDX21 plays vital roles in ribosomal RNA processing and the regulation of host innate immunity during virus infection. Here, we describe the optimized protocols for nucleic acid-free protein purification and crystallization of DDX21 in its different unwinding states. Rational design of...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420396/ https://www.ncbi.nlm.nih.gov/pubmed/36042885 http://dx.doi.org/10.1016/j.xpro.2022.101642 |