Imaging and analysis for simultaneous tracking of fluorescent biosensors in barcoded cells

We recently developed a biosensor barcoding approach for highly multiplexed tracking of molecular activities in live cells. In this protocol, we detail the labeling of cells expressing different genetically encoded fluorescent biosensors with a pair of barcoding proteins and parallel imaging. Signal...

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Detalles Bibliográficos
Autores principales: Chi, Wei-Yu, Au, Gabriel, Liang, Jessica, Chen, Chao-Cheng, Huang, Chuan-Hsiang, Yang, Jr-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420398/
https://www.ncbi.nlm.nih.gov/pubmed/36042884
http://dx.doi.org/10.1016/j.xpro.2022.101611

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420398/
https://www.ncbi.nlm.nih.gov/pubmed/36042884
http://dx.doi.org/10.1016/j.xpro.2022.101611

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