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Imaging and analysis for simultaneous tracking of fluorescent biosensors in barcoded cells
We recently developed a biosensor barcoding approach for highly multiplexed tracking of molecular activities in live cells. In this protocol, we detail the labeling of cells expressing different genetically encoded fluorescent biosensors with a pair of barcoding proteins and parallel imaging. Signal...
Autores principales: | Chi, Wei-Yu, Au, Gabriel, Liang, Jessica, Chen, Chao-Cheng, Huang, Chuan-Hsiang, Yang, Jr-Ming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420398/ https://www.ncbi.nlm.nih.gov/pubmed/36042884 http://dx.doi.org/10.1016/j.xpro.2022.101611 |
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