Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii

The auxin-inducible degron (AID) system is a useful technique to rapidly deplete any protein of interest “on-demand.” In this study, we successfully established the AID system for the “biotech” yeast Komagataella phaffii. First, we tested different expression levels of TIR1 for auxin-induced degrada...

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Detalles Bibliográficos
Autores principales: Lehmayer, Leonie, Bernauer, Lukas, Emmerstorfer-Augustin, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420516/
https://www.ncbi.nlm.nih.gov/pubmed/36043049
http://dx.doi.org/10.1016/j.isci.2022.104888

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420516/
https://www.ncbi.nlm.nih.gov/pubmed/36043049
http://dx.doi.org/10.1016/j.isci.2022.104888

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