Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes

The plasmid-free CRISPR-Cas9-based genome editing in fungi is a precise and time-saving approach. Here, we present a detailed protocol for genetic manipulation in Penicillium funiculosum, which includes design and synthesis of sgRNA, high-quality protoplast preparation, and PEG-mediated protoplast t...

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Detalles Bibliográficos
Autores principales: Sinha, Tulika, Yazdani, Syed Shams
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420531/
https://www.ncbi.nlm.nih.gov/pubmed/36042883
http://dx.doi.org/10.1016/j.xpro.2022.101629

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420531/
https://www.ncbi.nlm.nih.gov/pubmed/36042883
http://dx.doi.org/10.1016/j.xpro.2022.101629

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