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Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes

The plasmid-free CRISPR-Cas9-based genome editing in fungi is a precise and time-saving approach. Here, we present a detailed protocol for genetic manipulation in Penicillium funiculosum, which includes design and synthesis of sgRNA, high-quality protoplast preparation, and PEG-mediated protoplast t...

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Detalles Bibliográficos
Autores principales:	Sinha, Tulika, Yazdani, Syed Shams
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420531/ https://www.ncbi.nlm.nih.gov/pubmed/36042883 http://dx.doi.org/10.1016/j.xpro.2022.101629

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