Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids

Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering. We detail the differentiation of human intestinal organoids...

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Detalles Bibliográficos
Autores principales: Martinez-Silgado, Adriana, Yousef Yengej, Fjodor A., Puschhof, Jens, Geurts, Veerle, Boot, Charelle, Geurts, Maarten H., Rookmaaker, Maarten B., Verhaar, Marianne C., Beumer, Joep, Clevers, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420536/
https://www.ncbi.nlm.nih.gov/pubmed/36042877
http://dx.doi.org/10.1016/j.xpro.2022.101639

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9420536/
https://www.ncbi.nlm.nih.gov/pubmed/36042877
http://dx.doi.org/10.1016/j.xpro.2022.101639

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