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Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a

Detection of Burkholderia pseudomallei, a causative bacterium for melioidosis, remains a challenging undertaking due to long assay time, laboratory requirements, and the lack of specificity and sensitivity of many current assays. In this study, we are presenting a novel method that circumvents those...

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Detalles Bibliográficos
Autores principales: Wongpalee, Somsakul Pop, Thananchai, Hathairat, Chewapreecha, Claire, Roslund, Henrik B., Chomkatekaew, Chalita, Tananupak, Warunya, Boonklang, Phumrapee, Pakdeerat, Sukritpong, Seng, Rathanin, Chantratita, Narisara, Takarn, Piyawan, Khamnoi, Phadungkiat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423629/
https://www.ncbi.nlm.nih.gov/pubmed/36037185
http://dx.doi.org/10.1371/journal.pntd.0010659