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The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens

Plasmids that encode the same replication machinery are generally unable to coexist in the same bacterial cell. However, Clostridium perfringens strains often carry multiple conjugative toxin or antibiotic resistance plasmids that are closely related and encode similar Rep proteins. In many bacteria...

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Autores principales: Watts, Thomas D., Traore, Daouda A. K., Atkinson, Sarah C., Lao, Carmen, Caltabiano, Natalie, Rood, Julian I., Adams, Vicki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426499/
https://www.ncbi.nlm.nih.gov/pubmed/35726914
http://dx.doi.org/10.1128/mbio.01356-22
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author Watts, Thomas D.
Traore, Daouda A. K.
Atkinson, Sarah C.
Lao, Carmen
Caltabiano, Natalie
Rood, Julian I.
Adams, Vicki
author_facet Watts, Thomas D.
Traore, Daouda A. K.
Atkinson, Sarah C.
Lao, Carmen
Caltabiano, Natalie
Rood, Julian I.
Adams, Vicki
author_sort Watts, Thomas D.
collection PubMed
description Plasmids that encode the same replication machinery are generally unable to coexist in the same bacterial cell. However, Clostridium perfringens strains often carry multiple conjugative toxin or antibiotic resistance plasmids that are closely related and encode similar Rep proteins. In many bacteria, plasmid partitioning upon cell division involves a ParMRC system; in C. perfringens plasmids, there are approximately 10 different ParMRC families, with significant differences in amino acid sequences between each ParM family (15% to 54% identity). Since plasmids carrying genes belonging to the same ParMRC family are not observed in the same strain, these families appear to represent the basis for plasmid compatibility in C. perfringens. To understand this process, we examined the key recognition steps between ParR DNA-binding proteins and their parC binding sites. The ParR proteins bound to sequences within a parC site from the same ParMRC family but could not interact with a parC site from a different ParMRC family. These data provide evidence that compatibility of the conjugative toxin plasmids of C. perfringens is mediated by their parMRC-like partitioning systems. This process provides a selective advantage by enabling the host bacterium to maintain separate plasmids that encode toxins that are specific for different host targets.
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spelling pubmed-94264992022-08-31 The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens Watts, Thomas D. Traore, Daouda A. K. Atkinson, Sarah C. Lao, Carmen Caltabiano, Natalie Rood, Julian I. Adams, Vicki mBio Research Article Plasmids that encode the same replication machinery are generally unable to coexist in the same bacterial cell. However, Clostridium perfringens strains often carry multiple conjugative toxin or antibiotic resistance plasmids that are closely related and encode similar Rep proteins. In many bacteria, plasmid partitioning upon cell division involves a ParMRC system; in C. perfringens plasmids, there are approximately 10 different ParMRC families, with significant differences in amino acid sequences between each ParM family (15% to 54% identity). Since plasmids carrying genes belonging to the same ParMRC family are not observed in the same strain, these families appear to represent the basis for plasmid compatibility in C. perfringens. To understand this process, we examined the key recognition steps between ParR DNA-binding proteins and their parC binding sites. The ParR proteins bound to sequences within a parC site from the same ParMRC family but could not interact with a parC site from a different ParMRC family. These data provide evidence that compatibility of the conjugative toxin plasmids of C. perfringens is mediated by their parMRC-like partitioning systems. This process provides a selective advantage by enabling the host bacterium to maintain separate plasmids that encode toxins that are specific for different host targets. American Society for Microbiology 2022-06-21 /pmc/articles/PMC9426499/ /pubmed/35726914 http://dx.doi.org/10.1128/mbio.01356-22 Text en Copyright © 2022 Watts et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Watts, Thomas D.
Traore, Daouda A. K.
Atkinson, Sarah C.
Lao, Carmen
Caltabiano, Natalie
Rood, Julian I.
Adams, Vicki
The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title_full The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title_fullStr The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title_full_unstemmed The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title_short The Specificity of ParR Binding Determines the Incompatibility of Conjugative Plasmids in Clostridium perfringens
title_sort specificity of parr binding determines the incompatibility of conjugative plasmids in clostridium perfringens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9426499/
https://www.ncbi.nlm.nih.gov/pubmed/35726914
http://dx.doi.org/10.1128/mbio.01356-22
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