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Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression

BACKGROUND: Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South...

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Autores principales: Delgado-Diaz, David Jose, Jesaveluk, Brianna, Hayward, Joshua A., Tyssen, David, Alisoltani, Arghavan, Potgieter, Matthys, Bell, Liam, Ross, Elizabeth, Iranzadeh, Arash, Allali, Imane, Dabee, Smritee, Barnabas, Shaun, Gamieldien, Hoyam, Blackburn, Jonathan M., Mulder, Nicola, Smith, Steven B., Edwards, Vonetta L., Burgener, Adam D., Bekker, Linda-Gail, Ravel, Jacques, Passmore, Jo-Ann S., Masson, Lindi, Hearps, Anna C., Tachedjian, Gilda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429363/
https://www.ncbi.nlm.nih.gov/pubmed/36045402
http://dx.doi.org/10.1186/s40168-022-01337-5
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author Delgado-Diaz, David Jose
Jesaveluk, Brianna
Hayward, Joshua A.
Tyssen, David
Alisoltani, Arghavan
Potgieter, Matthys
Bell, Liam
Ross, Elizabeth
Iranzadeh, Arash
Allali, Imane
Dabee, Smritee
Barnabas, Shaun
Gamieldien, Hoyam
Blackburn, Jonathan M.
Mulder, Nicola
Smith, Steven B.
Edwards, Vonetta L.
Burgener, Adam D.
Bekker, Linda-Gail
Ravel, Jacques
Passmore, Jo-Ann S.
Masson, Lindi
Hearps, Anna C.
Tachedjian, Gilda
author_facet Delgado-Diaz, David Jose
Jesaveluk, Brianna
Hayward, Joshua A.
Tyssen, David
Alisoltani, Arghavan
Potgieter, Matthys
Bell, Liam
Ross, Elizabeth
Iranzadeh, Arash
Allali, Imane
Dabee, Smritee
Barnabas, Shaun
Gamieldien, Hoyam
Blackburn, Jonathan M.
Mulder, Nicola
Smith, Steven B.
Edwards, Vonetta L.
Burgener, Adam D.
Bekker, Linda-Gail
Ravel, Jacques
Passmore, Jo-Ann S.
Masson, Lindi
Hearps, Anna C.
Tachedjian, Gilda
author_sort Delgado-Diaz, David Jose
collection PubMed
description BACKGROUND: Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. RESULTS: Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. CONCLUSIONS: These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01337-5.
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spelling pubmed-94293632022-09-01 Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression Delgado-Diaz, David Jose Jesaveluk, Brianna Hayward, Joshua A. Tyssen, David Alisoltani, Arghavan Potgieter, Matthys Bell, Liam Ross, Elizabeth Iranzadeh, Arash Allali, Imane Dabee, Smritee Barnabas, Shaun Gamieldien, Hoyam Blackburn, Jonathan M. Mulder, Nicola Smith, Steven B. Edwards, Vonetta L. Burgener, Adam D. Bekker, Linda-Gail Ravel, Jacques Passmore, Jo-Ann S. Masson, Lindi Hearps, Anna C. Tachedjian, Gilda Microbiome Research BACKGROUND: Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. RESULTS: Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. CONCLUSIONS: These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01337-5. BioMed Central 2022-08-31 /pmc/articles/PMC9429363/ /pubmed/36045402 http://dx.doi.org/10.1186/s40168-022-01337-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Delgado-Diaz, David Jose
Jesaveluk, Brianna
Hayward, Joshua A.
Tyssen, David
Alisoltani, Arghavan
Potgieter, Matthys
Bell, Liam
Ross, Elizabeth
Iranzadeh, Arash
Allali, Imane
Dabee, Smritee
Barnabas, Shaun
Gamieldien, Hoyam
Blackburn, Jonathan M.
Mulder, Nicola
Smith, Steven B.
Edwards, Vonetta L.
Burgener, Adam D.
Bekker, Linda-Gail
Ravel, Jacques
Passmore, Jo-Ann S.
Masson, Lindi
Hearps, Anna C.
Tachedjian, Gilda
Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title_full Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title_fullStr Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title_full_unstemmed Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title_short Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
title_sort lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429363/
https://www.ncbi.nlm.nih.gov/pubmed/36045402
http://dx.doi.org/10.1186/s40168-022-01337-5
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