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CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO(2)-Fixing Methanogenic Archaeon

The CRISPR-Cas9 system is a robust genome editing tool that is widely applied in eukaryotes and bacteria. However, use of this technique has only been developed for one species of Archaea, a domain of life ranking in parallel with Eukarya and Bacteria. In this study, we applied the CRISPR-Cas9 genom...

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Detalles Bibliográficos
Autores principales: Li, Jie, Zhang, Liuyang, Xu, Qing, Zhang, Wenting, Li, Zhihua, Chen, Lei, Dong, Xiuzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430280/
https://www.ncbi.nlm.nih.gov/pubmed/35766512
http://dx.doi.org/10.1128/spectrum.01165-22