Closely related type II-C Cas9 orthologs recognize diverse PAMs

The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its targeting scope is limited by the protospacer-adjacent motif (PAM). To expand the target scope, it is crucial to develop a CRISPR toolbox capable of recognizing multiple PAMs. Here, using a GFP-activation assay, we test...

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Detalles Bibliográficos
Autores principales: Wei, Jingjing, Hou, Linghui, Liu, Jingtong, Wang, Ziwen, Gao, Siqi, Qi, Tao, Gao, Song, Sun, Shuna, Wang, Yongming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433092/
https://www.ncbi.nlm.nih.gov/pubmed/35959889
http://dx.doi.org/10.7554/eLife.77825

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433092/
https://www.ncbi.nlm.nih.gov/pubmed/35959889
http://dx.doi.org/10.7554/eLife.77825

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