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Closely related type II-C Cas9 orthologs recognize diverse PAMs

The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its targeting scope is limited by the protospacer-adjacent motif (PAM). To expand the target scope, it is crucial to develop a CRISPR toolbox capable of recognizing multiple PAMs. Here, using a GFP-activation assay, we test...

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Detalles Bibliográficos
Autores principales:	Wei, Jingjing, Hou, Linghui, Liu, Jingtong, Wang, Ziwen, Gao, Siqi, Qi, Tao, Gao, Song, Sun, Shuna, Wang, Yongming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2022
Materias:	Genetics and Genomics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433092/ https://www.ncbi.nlm.nih.gov/pubmed/35959889 http://dx.doi.org/10.7554/eLife.77825

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