R-loop formation and conformational activation mechanisms of Cas9

Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage(1–3). The programmable activity of Cas9 has been widely utilized for genome editing applications(4–6), yet its precise mechanisms of target DNA binding and off-target discrimination remain incompletely underst...

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Detalles Bibliográficos
Autores principales: Pacesa, Martin, Loeff, Luuk, Querques, Irma, Muckenfuss, Lena M., Sawicka, Marta, Jinek, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433323/
https://www.ncbi.nlm.nih.gov/pubmed/36002571
http://dx.doi.org/10.1038/s41586-022-05114-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433323/
https://www.ncbi.nlm.nih.gov/pubmed/36002571
http://dx.doi.org/10.1038/s41586-022-05114-0

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