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R-loop formation and conformational activation mechanisms of Cas9

Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage(1–3). The programmable activity of Cas9 has been widely utilized for genome editing applications(4–6), yet its precise mechanisms of target DNA binding and off-target discrimination remain incompletely underst...

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Detalles Bibliográficos
Autores principales:	Pacesa, Martin, Loeff, Luuk, Querques, Irma, Muckenfuss, Lena M., Sawicka, Marta, Jinek, Martin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433323/ https://www.ncbi.nlm.nih.gov/pubmed/36002571 http://dx.doi.org/10.1038/s41586-022-05114-0

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