Multicolor fluorescence activated cell sorting to generate humanized monoclonal antibody binding seven subtypes of BoNT/F

Generating specific monoclonal antibodies (mAbs) that neutralize multiple antigen variants is challenging. Here, we present a strategy to generate mAbs that bind seven subtypes of botulinum neurotoxin serotype F (BoNT/F) that differ from each other in amino acid sequence by up to 36%. Previously, we...

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Detalles Bibliográficos
Autores principales: Fan, Yongfeng, Sun, Zhengda, Conrad, Fraser, Wen, Weihua, Zhao, Lequn, Lou, Jianlong, Zhou, Yu, Farr-Jones, Shauna, Marks, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436041/
https://www.ncbi.nlm.nih.gov/pubmed/36048906
http://dx.doi.org/10.1371/journal.pone.0273512

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436041/
https://www.ncbi.nlm.nih.gov/pubmed/36048906
http://dx.doi.org/10.1371/journal.pone.0273512

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