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Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes

Poultry meat production is one of the most important agri‐food industries in the world. The selective pressure exerted by widespread prophylactic or therapeutic use of antibiotics in intensive chicken farming favours the development of drug resistance in bacterial populations. Chicken liver, closely...

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Autores principales: Blanco‐Picazo, Pedro, Gómez‐Gómez, Clara, Aguiló‐Castillo, Sergi, Fernández‐Orth, Dietmar, Cerdà‐Cuéllar, Marta, Muniesa, Maite, Rodríguez‐Rubio, Lorena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437878/
https://www.ncbi.nlm.nih.gov/pubmed/35485188
http://dx.doi.org/10.1111/1751-7915.14056
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author Blanco‐Picazo, Pedro
Gómez‐Gómez, Clara
Aguiló‐Castillo, Sergi
Fernández‐Orth, Dietmar
Cerdà‐Cuéllar, Marta
Muniesa, Maite
Rodríguez‐Rubio, Lorena
author_facet Blanco‐Picazo, Pedro
Gómez‐Gómez, Clara
Aguiló‐Castillo, Sergi
Fernández‐Orth, Dietmar
Cerdà‐Cuéllar, Marta
Muniesa, Maite
Rodríguez‐Rubio, Lorena
author_sort Blanco‐Picazo, Pedro
collection PubMed
description Poultry meat production is one of the most important agri‐food industries in the world. The selective pressure exerted by widespread prophylactic or therapeutic use of antibiotics in intensive chicken farming favours the development of drug resistance in bacterial populations. Chicken liver, closely connected with the intestinal tract, has been directly involved in food‐borne infections and found to be contaminated with pathogenic bacteria, including Campylobacter and Salmonella. In this study, 74 chicken livers, divided into sterile and non‐sterile groups, were analysed, not only for microbial indicators but also for the presence of phages and phage particles containing antibiotic resistance genes (ARGs). Both bacteria and phages were detected in liver tissues, including those dissected under sterile conditions. The phages were able to infect Escherichia coli and showed a Siphovirus morphology. The chicken livers contained from 10(3) to 10(6) phage particles per g, which carried a range of ARGs (bla (TEM), bla (CTx‐M‐1), sul1, qnrA, armA and tetW) detected by qPCR. The presence of phages in chicken liver, mostly infecting E. coli, was confirmed by metagenomic analysis, although this technique was not sufficiently sensitive to identify ARGs. In addition, ARG‐carrying phages were detected in chicken faeces by qPCR in a previous study of the group. Comparison of the viromes of faeces and liver showed a strong coincidence of species, which suggests that the phages found in the liver originate in faeces. These findings suggests that phages, like bacteria, can translocate from the gut to the liver, which may therefore constitute a potential reservoir of antibiotic resistance genes.
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spelling pubmed-94378782022-09-09 Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes Blanco‐Picazo, Pedro Gómez‐Gómez, Clara Aguiló‐Castillo, Sergi Fernández‐Orth, Dietmar Cerdà‐Cuéllar, Marta Muniesa, Maite Rodríguez‐Rubio, Lorena Microb Biotechnol Research Articles Poultry meat production is one of the most important agri‐food industries in the world. The selective pressure exerted by widespread prophylactic or therapeutic use of antibiotics in intensive chicken farming favours the development of drug resistance in bacterial populations. Chicken liver, closely connected with the intestinal tract, has been directly involved in food‐borne infections and found to be contaminated with pathogenic bacteria, including Campylobacter and Salmonella. In this study, 74 chicken livers, divided into sterile and non‐sterile groups, were analysed, not only for microbial indicators but also for the presence of phages and phage particles containing antibiotic resistance genes (ARGs). Both bacteria and phages were detected in liver tissues, including those dissected under sterile conditions. The phages were able to infect Escherichia coli and showed a Siphovirus morphology. The chicken livers contained from 10(3) to 10(6) phage particles per g, which carried a range of ARGs (bla (TEM), bla (CTx‐M‐1), sul1, qnrA, armA and tetW) detected by qPCR. The presence of phages in chicken liver, mostly infecting E. coli, was confirmed by metagenomic analysis, although this technique was not sufficiently sensitive to identify ARGs. In addition, ARG‐carrying phages were detected in chicken faeces by qPCR in a previous study of the group. Comparison of the viromes of faeces and liver showed a strong coincidence of species, which suggests that the phages found in the liver originate in faeces. These findings suggests that phages, like bacteria, can translocate from the gut to the liver, which may therefore constitute a potential reservoir of antibiotic resistance genes. John Wiley and Sons Inc. 2022-04-29 /pmc/articles/PMC9437878/ /pubmed/35485188 http://dx.doi.org/10.1111/1751-7915.14056 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Blanco‐Picazo, Pedro
Gómez‐Gómez, Clara
Aguiló‐Castillo, Sergi
Fernández‐Orth, Dietmar
Cerdà‐Cuéllar, Marta
Muniesa, Maite
Rodríguez‐Rubio, Lorena
Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title_full Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title_fullStr Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title_full_unstemmed Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title_short Chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
title_sort chicken liver is a potential reservoir of bacteriophages and phage‐derived particles containing antibiotic resistance genes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437878/
https://www.ncbi.nlm.nih.gov/pubmed/35485188
http://dx.doi.org/10.1111/1751-7915.14056
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