Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes

The ability of T7 RNA polymerase (RNAP(T7)) fusions to cytosine deaminases (CdA) for entering C➔T changes in any DNA segment downstream of a T7 promoter was exploited for hyperdiversification of defined genomic portions of Pseudomonas putida KT2440. To this end, test strains were constructed in whic...

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Detalles Bibliográficos
Autores principales: Velázquez, Elena, Álvarez, Beatriz, Fernández, Luis Ángel, de Lorenzo, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437889/
https://www.ncbi.nlm.nih.gov/pubmed/35695013
http://dx.doi.org/10.1111/1751-7915.14098

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437889/
https://www.ncbi.nlm.nih.gov/pubmed/35695013
http://dx.doi.org/10.1111/1751-7915.14098

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