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Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes
The ability of T7 RNA polymerase (RNAP(T7)) fusions to cytosine deaminases (CdA) for entering C➔T changes in any DNA segment downstream of a T7 promoter was exploited for hyperdiversification of defined genomic portions of Pseudomonas putida KT2440. To this end, test strains were constructed in whic...
Autores principales: | Velázquez, Elena, Álvarez, Beatriz, Fernández, Luis Ángel, de Lorenzo, Víctor |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437889/ https://www.ncbi.nlm.nih.gov/pubmed/35695013 http://dx.doi.org/10.1111/1751-7915.14098 |
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