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Be aware of the allele-specific bias and compositional effects in multi-template PCR

High-throughput sequencing of amplicon libraries is the most widespread and one of the most effective ways to study the taxonomic structure of microbial communities, even despite growing accessibility of whole metagenome sequencing. Due to the targeted amplification, the method provides unparalleled...

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Detalles Bibliográficos
Autores principales: Korvigo, Ilia, Igolkina, Anna A., Kichko, Arina A., Aksenova, Tatiana, Andronov, Evgeny E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438772/
https://www.ncbi.nlm.nih.gov/pubmed/36061756
http://dx.doi.org/10.7717/peerj.13888