Genome editing in the edible fungus Poria cocos using CRISPR-Cas9 system integrating genome-wide off-target prediction and detection

Poria cocos is an important edible and medicinal fungus with a long history. However, the lack of adequate genetic tools has hindered molecular genetic research and the genetic modification of this species. In this study, the endogenous U6 promoters were identified by mining data from the P. cocos g...

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Detalles Bibliográficos
Autores principales: Xie, Zhenni, Zhong, Can, Liu, Xiaoliu, Wang, Ziling, Zhou, Rongrong, Xie, Jing, Zhang, Shuihan, Jin, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441760/
https://www.ncbi.nlm.nih.gov/pubmed/36071963
http://dx.doi.org/10.3389/fmicb.2022.966231

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441760/
https://www.ncbi.nlm.nih.gov/pubmed/36071963
http://dx.doi.org/10.3389/fmicb.2022.966231

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