Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling

Translational regulation is a fundamental step in gene expression with critical roles in biological processes within a cell. Here, we describe a protocol to assess translation activity in mammalian cells by incorporation of O-propargyl-puromycin (OP-Puro). OP-Puro is a puromycin analog that is incor...

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Detalles Bibliográficos
Autores principales: Hsu, Jack Chun-Chieh, Pawlak, Joanna B., Laurent-Rolle, Maudry, Cresswell, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442383/
https://www.ncbi.nlm.nih.gov/pubmed/36072758
http://dx.doi.org/10.1016/j.xpro.2022.101654

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442383/
https://www.ncbi.nlm.nih.gov/pubmed/36072758
http://dx.doi.org/10.1016/j.xpro.2022.101654

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