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Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Translational regulation is a fundamental step in gene expression with critical roles in biological processes within a cell. Here, we describe a protocol to assess translation activity in mammalian cells by incorporation of O-propargyl-puromycin (OP-Puro). OP-Puro is a puromycin analog that is incor...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442383/ https://www.ncbi.nlm.nih.gov/pubmed/36072758 http://dx.doi.org/10.1016/j.xpro.2022.101654 |