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In situ single particle classification reveals distinct 60S maturation intermediates in cells

Previously, we showed that high-resolution template matching can localize ribosomes in two-dimensional electron cryo-microscopy (cryo-EM) images of untilted Mycoplasma pneumoniae cells with high precision (Lucas et al., 2021). Here, we show that comparing the signal-to-noise ratio (SNR) observed wit...

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Detalles Bibliográficos
Autores principales: Lucas, Bronwyn A, Zhang, Kexin, Loerch, Sarah, Grigorieff, Nikolaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9444246/
https://www.ncbi.nlm.nih.gov/pubmed/36005291
http://dx.doi.org/10.7554/eLife.79272