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SAIBR: a simple, platform-independent method for spectral autofluorescence correction

Biological systems are increasingly viewed through a quantitative lens that demands accurate measures of gene expression and local protein concentrations. CRISPR/Cas9 gene tagging has enabled increased use of fluorescence to monitor proteins at or near endogenous levels under native regulatory contr...

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Detalles Bibliográficos
Autores principales: Rodrigues, Nelio T. L., Bland, Tom, Borrego-Pinto, Joana, Ng, KangBo, Hirani, Nisha, Gu, Ying, Foo, Sherman, Goehring, Nathan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445497/
https://www.ncbi.nlm.nih.gov/pubmed/35713287
http://dx.doi.org/10.1242/dev.200545