Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR screening

CRISPR technology is a powerful tool for studying genome function. To aid in picking sgRNAs that have maximal efficacy against a target of interest from many possible options, several groups have developed models that predict sgRNA on-target activity. Although multiple tracrRNA variants are commonly...

Descripción completa

Detalles Bibliográficos
Autores principales: DeWeirdt, Peter C., McGee, Abby V., Zheng, Fengyi, Nwolah, Ifunanya, Hegde, Mudra, Doench, John G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448816/
https://www.ncbi.nlm.nih.gov/pubmed/36068235
http://dx.doi.org/10.1038/s41467-022-33024-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448816/
https://www.ncbi.nlm.nih.gov/pubmed/36068235
http://dx.doi.org/10.1038/s41467-022-33024-2

Ejemplares similares