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Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR screening

CRISPR technology is a powerful tool for studying genome function. To aid in picking sgRNAs that have maximal efficacy against a target of interest from many possible options, several groups have developed models that predict sgRNA on-target activity. Although multiple tracrRNA variants are commonly...

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Detalles Bibliográficos
Autores principales:	DeWeirdt, Peter C., McGee, Abby V., Zheng, Fengyi, Nwolah, Ifunanya, Hegde, Mudra, Doench, John G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448816/ https://www.ncbi.nlm.nih.gov/pubmed/36068235 http://dx.doi.org/10.1038/s41467-022-33024-2

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