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Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells

Endometriosis is characterized by the presence of inflamed and fibrotic endometrial tissue outside the uterine cavity. Previously, we found decreased SERPINA1 (alpha-1 antitrypsin) expression in endometriosis-like lesions in a mouse model of endometriosis, suggesting that it exacerbated inflammation...

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Autores principales: Kusama, Kazuya, Satoyoshi, Ayaka, Azumi, Mana, Yoshie, Mikihiro, Kojima, Junya, Mizuno, Yumi, Ono, Masanori, Nishi, Hirotaka, Kajihara, Takeshi, Tamura, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448891/
https://www.ncbi.nlm.nih.gov/pubmed/36093086
http://dx.doi.org/10.3389/fendo.2022.966455
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author Kusama, Kazuya
Satoyoshi, Ayaka
Azumi, Mana
Yoshie, Mikihiro
Kojima, Junya
Mizuno, Yumi
Ono, Masanori
Nishi, Hirotaka
Kajihara, Takeshi
Tamura, Kazuhiro
author_facet Kusama, Kazuya
Satoyoshi, Ayaka
Azumi, Mana
Yoshie, Mikihiro
Kojima, Junya
Mizuno, Yumi
Ono, Masanori
Nishi, Hirotaka
Kajihara, Takeshi
Tamura, Kazuhiro
author_sort Kusama, Kazuya
collection PubMed
description Endometriosis is characterized by the presence of inflamed and fibrotic endometrial tissue outside the uterine cavity. Previously, we found decreased SERPINA1 (alpha-1 antitrypsin) expression in endometriosis-like lesions in a mouse model of endometriosis, suggesting that it exacerbated inflammation in these lesions. However, the molecular mechanism(s) by which SERPINA1 affects expression of inflammatory factors and development of endometriotic lesions have not been fully characterized. To investigate the role of intracellular SERPINA1 in endometrial stromal cells (ESCs), we performed RNA sequence analysis using RNA extracted from ESCs in which SERPINA1 was knocked down. The analysis identified several toll-like receptor (TLR)-related factors as being upregulated. Silencing of SERPINA1 increased expression of TLR3 and TLR4 in ESCs, as well as several TLR signaling pathway components, including MYD88, IRAK1/4, interleukin (IL)-1β, and interferon (IFN)-β. TLR3 or TLR4 agonists increased expression of inflammatory factors in SERPINA1-knockdown ESCs, whereas TLR3 or TLR4 inhibitors decreased expression. In addition, treatment with recombinant IL-1β or IFN-β increased expression of MYD88 and inflammatory factors in ESCs. Immunohistochemical analysis of endometriotic tissues showed that TLR3, TLR4, and MYD88 were localized in endometriosis lesions. Taken together, the data suggest that reduced expression of SERPINA1 induces expression of inflammatory factors by ESCs, which in turn are associated with TLR3/4, IL-1β, and IFN-β signaling. Regulation of intracellular SERPINA1 levels in ESCs may be a strategy to inhibit inflammatory responses in endometriotic lesions.
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spelling pubmed-94488912022-09-08 Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells Kusama, Kazuya Satoyoshi, Ayaka Azumi, Mana Yoshie, Mikihiro Kojima, Junya Mizuno, Yumi Ono, Masanori Nishi, Hirotaka Kajihara, Takeshi Tamura, Kazuhiro Front Endocrinol (Lausanne) Endocrinology Endometriosis is characterized by the presence of inflamed and fibrotic endometrial tissue outside the uterine cavity. Previously, we found decreased SERPINA1 (alpha-1 antitrypsin) expression in endometriosis-like lesions in a mouse model of endometriosis, suggesting that it exacerbated inflammation in these lesions. However, the molecular mechanism(s) by which SERPINA1 affects expression of inflammatory factors and development of endometriotic lesions have not been fully characterized. To investigate the role of intracellular SERPINA1 in endometrial stromal cells (ESCs), we performed RNA sequence analysis using RNA extracted from ESCs in which SERPINA1 was knocked down. The analysis identified several toll-like receptor (TLR)-related factors as being upregulated. Silencing of SERPINA1 increased expression of TLR3 and TLR4 in ESCs, as well as several TLR signaling pathway components, including MYD88, IRAK1/4, interleukin (IL)-1β, and interferon (IFN)-β. TLR3 or TLR4 agonists increased expression of inflammatory factors in SERPINA1-knockdown ESCs, whereas TLR3 or TLR4 inhibitors decreased expression. In addition, treatment with recombinant IL-1β or IFN-β increased expression of MYD88 and inflammatory factors in ESCs. Immunohistochemical analysis of endometriotic tissues showed that TLR3, TLR4, and MYD88 were localized in endometriosis lesions. Taken together, the data suggest that reduced expression of SERPINA1 induces expression of inflammatory factors by ESCs, which in turn are associated with TLR3/4, IL-1β, and IFN-β signaling. Regulation of intracellular SERPINA1 levels in ESCs may be a strategy to inhibit inflammatory responses in endometriotic lesions. Frontiers Media S.A. 2022-08-24 /pmc/articles/PMC9448891/ /pubmed/36093086 http://dx.doi.org/10.3389/fendo.2022.966455 Text en Copyright © 2022 Kusama, Satoyoshi, Azumi, Yoshie, Kojima, Mizuno, Ono, Nishi, Kajihara and Tamura https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Kusama, Kazuya
Satoyoshi, Ayaka
Azumi, Mana
Yoshie, Mikihiro
Kojima, Junya
Mizuno, Yumi
Ono, Masanori
Nishi, Hirotaka
Kajihara, Takeshi
Tamura, Kazuhiro
Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title_full Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title_fullStr Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title_full_unstemmed Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title_short Toll-like receptor signaling pathway triggered by inhibition of serpin A1 stimulates production of inflammatory cytokines by endometrial stromal cells
title_sort toll-like receptor signaling pathway triggered by inhibition of serpin a1 stimulates production of inflammatory cytokines by endometrial stromal cells
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448891/
https://www.ncbi.nlm.nih.gov/pubmed/36093086
http://dx.doi.org/10.3389/fendo.2022.966455
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