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Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
BACKGROUND: The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair over non...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450370/ https://www.ncbi.nlm.nih.gov/pubmed/36071435 http://dx.doi.org/10.1186/s12934-022-01908-z |